PROFILE TOTAL POLYPHENOLOF THE ETHANOL EXTRACT FROM DENGEN (Dilleniaserrata) LEAF AND STEM BARK WHICH COMES FROM MALANGKE CITY LUWU DISTRICT

Santi Sinala, Ismail Ibrahim, Alfrida Monica Salasa

Abstract


Plant contain metabolite Secondary various kind of type . Product waste from plant this on the principle could made into to be medicine. In the urban era now this , more degenerative diseases suffered by society . Diseases that including diabetes, disease heart , cancer and aging early. Diseases this caused by the presence of ROS (reactive Oxygen Species ) or usually called free radical. Dengen have long been used by the community as medicine. The fruit is used as an enhancer of sour taste in dishes while the stem bark is boiled and orally as a treatment for vomiting blood.Based on research, this fruit contains vitamin C more than 84%, the ethanol extract of Dengen fruit contains tannin, saponins , alkaloid compounds, flavonoids, polyphenols and triterpenoids.The aim of this research was to determinethetotalpolyphenolsfromethanol extract leafandstem bark of Dengen(Dilleniaserrata).Leafandstem bark extracted by macerationby usingethanol 70%.Ethanol extract of leafmadeinconcentration of500µg/ml.Whileethanol extract of stem bark madeinconcentration of100ug/mlwithadditionsFolin-Coicalteau (1:10) and Na2CO37.5%.Absorbance of samplebe measuredusespectrophotometerUV-Visat a wavelength of745nm.The measurement results are based on absorban color intensity calibrated in the raw curve of gallicacid.Data analysis using the standard curve equationy = 0.0119x + 0.0416.The results obtained where the content of polyphenols in ethanol extract of dengen leaves were 39,793 mgGAE/g or 3.97% b/b while those in stem bark were 444.8 mgGAE /g or 44.8% b/b.

Keywords : Total Polyphenol, Ethanol Extract, Leaves of Dengen, Stem Bark of Dengen (Dilleniaserrata), Gallat Acid, Spectrophotometer UV VIS

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DOI: https://doi.org/10.32382/uh.v2i1.1181

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