COMPATIBILITY TEST DIAGNOSTIC KITS OF NONSTRUCTURAL-1 DENGUE ANTIGEN IMMUNOCROMATOGRAPHY METHOD OF PATIENT’S SERUM WITH SUSPECT DENGUE HAEMORRHAGIC FEVER

Yaumil Fachni Tandjungbulu, Zulfikar Ali Hasan, Haerani Haerani

Abstract


This study aims to determine the comparison of diagnostic kit test results of the Nonstructural-1 dengue antigen which is currently being developed with Nonstructural-1 dengue antigen commercial kit used in hospitals using methods Immunocromatography of patient’s serum withDHF suspect. The study design used a cross-sectional method with a total one hundred and three samples that met the inclusion criteria. Collection and examination of samples were carried out at the Pathology Laboratory of the Education Hospital of Hasanuddin University Makassar during March to June 2018. The results showed that the examination using the development NS1 kit obtained positive results as many as 56 samples and negative results as many as 47 samples, while the examination using a commercial NS1 kit obtained positive results as many as 51 samples and negative results as many as 52 samples. The Cohen's Kappa test results obtained a value of p=0,000 (<0.05) indicating that there was a significant match between the development NS1 kit and the commercial NS1 kit used in the hospital and had a very good degree of suitability because of the Cohen's Kappa value obtained in the research is equal to K=0.903 (>0.75). In this study, the sensitivity level of NS1 kit development was 100%, specificity was 90.4%, positive predictive value was 91.1%, negative predictive value was 100%, and the accuracy value was 95.1%.

Keywords: NS1 Dengue Kit, Immunochromatography Method, Dengue Hemorrhagic Fever


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References


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DOI: https://doi.org/10.32382/uh.v2i1.1402

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