KONFIRMASI HASIL OSMOTIC FRAGILITY TEST (OFT) BERDASARKAN KADAR HEMOGLOBIN

Widya Cahya Maggyasari; Budi Santosa

doi:10.32382/mak.v13i2.2883

KONFIRMASI HASIL OSMOTIC FRAGILITY TEST (OFT) BERDASARKAN KADAR HEMOGLOBIN

Widya Cahya Maggyasari, Budi Santosa

Abstract

Fragilitas eritrosit merupakan reaksi membran eritrosit untuk melawan tekanan osmosis larutan disekitarnya. Beberapa faktor yang mempengaruhi adalah kadar hemoglobin, bentuk eritrosit, umur eritrosit, kandungan lipoprotein dinding eritrosit. Tujuan penelitian untuk mengetahui hasil osmotic fragility test (OFT) bedasarkan kadar hemoglobin. Metode penelitian deskriftif dengan populasi mahasiswa analis kesehatan Unimus. Sampel sebanyak 20 orang dan spesimen berupa darah vena yang diperiksa kadar Hb menggunakan alat hematology analyzer dan kadar OFT menggnakan seri pengenceran tabung reaksi. Hasil penelitian menunjukkan rerata kadar OFT 0,37±1,67 dan kadar Hb 13,39 ±0,01. Terjadi lisis sempurna dengan kadar OFT 0,46% pada kadar Hb ≥ 15 g/dl. Simpulan kadar OFT dan kadar Hb reratanya masih dalam batas normal, ada 4 kadar Hb dibawah normal atau anemia ringan yang memiliki kadar OFT normal, sedangakan kadar Hb ≥ 15 g/dl memiliki kadar OFT diatas normal.Fragilitas eritrosit merupakan reaksi membran eritrosit untuk melawan tekanan osmosis larutan disekitarnya. Beberapa faktor yang mempengaruhi adalah kadar hemoglobin, bentuk eritrosit, umur eritrosit, kandungan lipoprotein dinding eritrosit. Tujuan penelitian untuk mengetahui hasil osmotic fragility test (OFT) bedasarkan kadar hemoglobin. Metode penelitian deskriftif dengan populasi mahasiswa analis kesehatan Unimus. Sampel sebanyak 20 orang dan spesimen berupa darah vena yang diperiksa kadar Hb menggunakan alat hematology analyzer dan kadar OFT menggnakan seri pengenceran tabung reaksi. Hasil penelitian menunjukkan rerata kadar OFT 0,37±1,67 dan kadar Hb 13,39 ±0,01. Terjadi lisis sempurna dengan kadar OFT 0,46% pada kadar Hb ≥ 15 g/dl. Simpulan kadar OFT dan kadar Hb reratanya masih dalam batas normal, ada 4 kadar Hb dibawah normal atau anemia ringan yang memiliki kadar OFT normal, sedangakan kadar Hb ≥ 15 g/dl memiliki kadar OFT diatas normal.

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References

Agus, R. T. A. (2017). Prototype alat pendeteksi kadar oksigen dalam darah (hemoglobin/hb) menggunakan mikrokontroler atmega 8535. Jurteksi royal Vol 3 No 2, 3.

Aldrich, K. J. (2012). Investigation of factors influencing erythrocyte osmotic fragility among selected ectothermic and endothermic vertegrates.

Amalia, D. (2021). Teknik Data Mining pada Prediksi Klasifikasi Jenis Penyakit Anemia. Universitas Multimedia Nusantara.

Antwi-Baffour, S., Quao, E., Kyeremeh, R., & Mahmood, S. A. (2013). Prolong storage of blood in EDTA has an effect on the morphology and osmotic fragility of erythrocytes. Int J Biomed Sci Eng, 1(2), 20–23.

Arora, R. D., Dass, J., Maydeo, S., Arya, V., Radhakrishnan, N., Sachdeva, A., Kotwal, J., & Bhargava, M. (2018). Flow cytometric osmotic fragility test and eosin‐5’‐maleimide dye‐binding tests are better than conventional osmotic fragility tests for the diagnosis of hereditary spherocytosis. International Journal of Laboratory Hematology, 40(3), 335–342.

Atmaca, N., Cinar, M., Guner, B., Kabakci, R., Gazyagci, A. N., Atmaca, H. T., & Canpolat, S. (2015). Evaluation of oxidative stress, hematological and biochemical parameters during Toxoplasma gondii infection in gerbils.

Barcellini, W., & Fattizzo, B. (2015). Clinical applications of hemolytic markers in the differential diagnosis and management of hemolytic anemia. Disease Markers, 2015.

Brodsky, R. A. (2015). Complement in hemolytic anemia. Blood, The Journal of the American Society of Hematology, 126(22), 2459–2465.

Cappellini, M. D., & Motta, I. (2015). Anemia in clinical practice—definition and classification: does hemoglobin change with aging? Seminars in Hematology, 52(4), 261–269.

da Silva Garrote-Filho, M., Bernardino-Neto, M., & Penha-Silva, N. (2017). Influence of erythrocyte membrane stability in atherosclerosis. Current Atherosclerosis Reports, 19(4), 1–7.

Demirkaya, K., Demirdöğen, B. C., Torun, Z. Ö., Erdem, O., & Tunca, Y. M. (2018). The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver. Turkish Journal of Biochemistry, 43(3), 333–341.

Ellsworth, M. L., & Sprague, R. S. (2012). Regulation of blood flow distribution in skeletal muscle: role of erythrocyte‐released ATP. The Journal of Physiology, 590(20), 4985–4991.

Gérard, D., Fattet, A., Brakta, C., Phulpin, A., Steschenko, D., Lesesve, J., & Perrin, J. (2017). Evaluation of OSMOCELLS, a new semi‐automatic device for osmotic fragility assessment. International Journal of Laboratory Hematology, 39(5), 521–527.

Goodnough, L. T., & Schrier, S. L. (2014). Evaluation and management of anemia in the elderly. American Journal of Hematology, 89(1), 88–96.

Gyawali, P., Richards, R. S., Hughes, D. L., & Tinley, P. (2014). Erythrocyte aggregation and metabolic syndrome. Clinical Hemorheology and Microcirculation, 57(1), 73–83.

Haley, K. (2017). Congenital hemolytic anemia. Medical Clinics, 101(2), 361–374.

Harvey, J. W. (2022). Erythrocyte biochemistry. Schalm’s Veterinary Hematology, 166–171.

Igbokwe, N. A. (2018). A review of the factors that influence erythrocyte osmotic fragility. Sokoto Journal of Veterinary Sciences, 16(4), 1–23.

Phillips, J., & Henderson, A. C. (2018). Hemolytic anemia: evaluation and differential diagnosis. American Family Physician, 98(6), 354–361.

Rismayanthi, C. (2016). Profil tingkat volume oksigen maskimal (VO2 max) dan kadar hemoglobin (HB) pada atlet Yongmoodo Akademi Militer Magelang. Jorpres (Jurnal Olahraga Prestasi), 12(2).

Rodríguez-Varela, M., García-Rubio, D., De la Mora-Mojica, B., Méndez-Méndez, J., Durán-Álvarez, C., & Cerecedo, D. (2019). Alterations to plasma membrane lipid contents affect the biophysical properties of erythrocytes from individuals with hypertension. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1861(10), 182996.

Salvagno, G. L., Demonte, D., Dima, F., Bovo, C., & Lippi, G. (2020). Stability of refrigerated whole blood samples for osmotic fragility test. Hematology, Transfusion and Cell Therapy, 42, 134–138.

Tritschler, C., Mizukami, K., Raj, K., & Giger, U. (2016). Increased erythrocytic osmotic fragility in anemic domestic shorthair and purebred cats. Journal of Feline Medicine and Surgery, 18(6), 462–470.

Tvedten, H. (2022). Classification and laboratory evaluation of anemia. Schalm’s Veterinary Hematology, 198–208.

Zhang, L., HU, Z., Pan, J., WU, P., Zhou, R., & Zhu, Q. (2015). Discussion of Improved Examination Method of Osmotic Fragility Test. Journal of Modern Laboratory Medicine, 94–97.

DOI: https://doi.org/10.32382/mak.v13i2.2883