SENSITIVITAS Streptococcus pneumoniae, Staphylococcus aureus dan Staphylococcus epidermidis terhadap BUAH ASAM JAWA (Tamarindus indica L)

Sesilia Rante Pakadang, Hiany Salim Salim

Abstract


Sputum cough is a common manifestation of URI (Upper Respiratory Tract Infection). Streptococcus pneumoniae, Staphylococcus aureus, and Staphylococcus epidermidis are common bacteria isolated from the phlegm of patients with URI. This determines the potential of the tamarind juice extract on Streptococcus pneumoniae, Staphylococcus aureus, and Staphylococcus epidermidis. Also, the study focuses on the bacteria that are sensitive to the Javanese tamarind fruit. Testing is carried out in vitro using the diffusion method with MHA media. Measurement of bacterial growth inhibition is carried out after 1x24 hours of incubation. The results were determined based on the mean of inhibition (mm) of bacterial growth in a row for SPBAJ test material concentrations of 10%, 20%, 40%, amoxicillin 50 ppm, and aqua pro injection. Streptococcus pneumoniae had 12.3; 13.7; 16.7; and 9; 0, Staphylococcus aureus 10.7; 13; 15; and 11; 0, and Staphylococcus epidermidis 11.3; 13.7; 14.3, and 14.3; 0. The results show that Java Tamarind Juice has the potential to inhibit the growth of Streptococcus pneumoniae bacteria. Also, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pneumoniae are sensitive to the Java tamarind fruit.

Keywords: Java tamarind fruit, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis

Batuk berdahak merupakan manifestasi umum dari penyakit ISPA. Streptococcus pneumoniae, Staphylococcus aureus dan Staphylococcus epidermidis adalah beberapa bakteri yang berhasil diisolasi dari lendir dahak penderita ISPA. Tujuan penelitian ini adalah menentukan potensi dari sari perasan buah asam jawa (Tamarindus indica L) terhadap Streptococcus pneumoniae, Staphylococcus aureus dan Staphylococcus epidermidis dan menentukan bakteri yang palling sensitivitas terhadap buah asam jawa. Metode penelitian:  bahan uji sari perasan buah asam jawa (SPBAJ)  disiapkan dengan konsentrasi 10, 20 dan 40%,control  amoksisilin 50 ppm dan aqua pro injeksi. Pengujian dilakukan secara in vitro dengan metode difusi agar menggunakan media MHA. Pengukuran daya hambat pertumbuhan bakteri dilakukan setelah inkubasi 1x24 jam.   Hasil penelitian  menunjukkan bahwa rerata daya hambat (mm) pertumbuhan bakteri berturut-turut untuk bahan uji SPBAJ konsentrasi 10%, 20%, 40%, amoksisilin 50 ppm dan aqua pro injeksi: untuk Streptococcus pneumoniae adalah 12,3; 13,7; 16,7; 9; 0. Untuk Staphylococcus aureus adalah 10,7; 13; 15; 11; 0. Untuk  Staphylococcus epidermidis adalah 11,3; 13,7; 14,3; 14,3; 0. Kesimpulan; Sari perasan buah asam jawa (Tamarindus indica L) berpotensi menghambat pertumbuhan bakteri  Streptococcus pneumoniae, Staphylococcus aureus dan Staphylococcus epidermidis (hasil isolat dahak penderita ISPA) dan Streptococcus pneumoniae adalah bakteri yang palling sensitivitas terhadap buah asam jawa.

Kata kunci : buah asam jawa, Streptococcus pneumoniae, Staphylococcus aureus,  Staphylococcus epidermidis


References


Ahmed dan Bahar. 2007. Chemistry Of Natural Products. New Delhi:Department of Pharmaceutical Chemistry Faculty of Science Jamia Hamdard. India.

Chibane L.B., Forquet P., Clement Y., Akkari L.L., Oulahal N., Degraeve P., Borges C., 2019. Antibacterial Properties of Polyphenols: Characterization and QSAR (Quantitative Structure–Activity Relationship) Models. Front Microbiol. 2019; 10: 829. Published online 2019 Apr 18. doi: 10.3389/fmicb.2019.00829. PMCID: PMC6482321. PMID: 31057527

Cushnie T. dan Lamb A.J., 2005. Antimicrobial activity of flavonoids International Journal of Antimicrobial Agents 26(5):343-56. DOI: 10.1016/j.ijantimicag.2005.09.002 Source PubMed

Darsana I., Besung I., Mahatmi H. 2012. Potensi Daun Binahong (Anredera Cordifolia (Tenore) Steenis) dalam Menghambat Pertumbuhan Bakteri Escherichia coli secara In Vitro. Indonesia Medicus Veterinus.2012.

Doughari J.H. 2006. Antimicrobial Activity of Tamarindus indica Linn, Tropical Journal of Pharmaceutical Research, 5(2) ; 597-603.

Hernani. 2011. Pengembangan Biofarmaka Sebagai Obat Herbal Untuk Kesehatan. Buletin Teknologi Pascapanen Pertanian. 2011; 7(1): 20-29.

Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. 2011. Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci. 2011;12: 3422-3431

Karou D. Dan Savadogo A. 2005. Antibacterial activity of alkaloids from Sida acuta. African Journal of Biotechnology. 2005.4(12): 1452-1457.

Kementerian Kesehatan Republik Indonesia. 2013. Riset Kesehatan Dasar. Jakarta: Kemenkes RI.

Khan M.I., Ahhmed A., Shin J.H., Baek J.S, Kim M.Y., Kim J.D., 2018. Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram Negative Bacteria, a Comprehensive Study In Vitro and In Vivo. Hindawi. Evidence-Based Complementary and Alternative Medicine :Volume 2018, Article ID 3486106, 12 pages https://doi.org/10.1155/2018/3486106

Mabhiza D., Chitemerere T., Mukanganyama S. 2016. Research Article Antibacterial Properties of Alkaloid Extracts from Callistemon citrinus and Vernonia adoensis against Staphylococcus aureus and Pseudomonas aeruginosa. Hindawi Publishing Corporation International Journal of Medicinal Chemistry Volume 2016, Article ID 6304163, 7 pages http://dx.doi.org/10.1155/2016/6304163.

Madduluri S., Rao K.B., Sitaram B. 2013. In Vitro Evaluation of Antibacterial Activity of Five Indegenous Plants Extract Against Five Bacterial Pathogens of Human. International Journal of Pharmacy and Pharmaceutical Sciences.2013:5(4): 679-684.

Nuria M.C., Faizaitun A.S. 2009. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas L) Terhadap Bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408, Mediagro. 2009; 5(2): 26–37.

Pakadang S.R. dan Salim H. 2019. Kombinasi Daun Miana (Coleus scutellarioides (L.) Benth) dan Rimpang Jahe (Zingiber officinale Rosc.) sebagai antibakteri Streptococcus pneumonia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia Penyebab Batuk. Media Farmasi P.ISSN 0216-2083. E ISSN 2622-0962 vol XV no. 1, April 2019. DOI: https://doi.org/10.32382/mf.v15i1.779

Pakadang S.R., 2018. Potential of Miana Leaves (Coleus scutellarioides (L.) Benth) As an Antibacterial Streptococcus pneumonia, Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia from Sputum Cough Patients in Makassar City. Proceeding 1st. International Conference Health Polytechnic of Kupang, 122-131. Retrieved from http://proceeding.poltekeskupang.ac.id/index.php/ichpk/article/view/45

Panggalo, TJ., Porotu’o, J., dan Buntuan, V. 2013. “ Identifikasi Bakteri Aerob Pada Penderita Batuk Berdahak Di Poliklinik Interna BLU RSUP Prof. dr. R. D. Kandou Manado”. Jurnal e-Biomedik Volume 1 Nomor 1, hlm. 408-413.

Poongothai P. Dan Rajan S. 2013. Antibacterial Properties of Mangifera indica flower extracts on Urophatogenic Escherichia coli,.International Journal of Current Microbiology and Aplied Science. 2013; 2(12): 104-111

Puspodewi. 2015. Daya hambat daun Asam Jawa (Tamarindus indica L) Terhadap Pertumbuhan Salmonella typhi Penyebab Demam tifoid. Skripsi. unimus press.semarang.

Sari, F.P. dan S. M. Sari. 2011. Ekstraksi Zat Aktif Antimikroba dari Tanaman Yodium (Jatropha multifida Linn) sebagai Bahan Baku Alternatif Antibiotik Alami. Skripsi. Fakultas Teknik Universitas Diponegoro. Semarang.

World Health Organization. 2008. Epidemic-prone & pandemic-prone acute respiratory diseases: Infection prevention & control in health-care facilities. Summary guidance 2007 WHO/CDS/EPR/2007.8 © World Health Organization. Jenewa.




DOI: https://doi.org/10.32382/mf.v16i1.1407

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