Uji Efek Antioksidan Formulasi Sediaan Sirup Ekstrak Etanol Kulit Buah Jeruk Bali (Citrus Maxima Merr.) Terhadap Malondialdehid Tikus Putih (Rattus Novergicus) Yang Diinduksi Karbon Tetraklorida

Suryanita Suryanita; Hasma Hasma

doi:10.32382/medkes.v16i1.1836

Uji Efek Antioksidan Formulasi Sediaan Sirup Ekstrak Etanol Kulit Buah Jeruk Bali (Citrus Maxima Merr.) Terhadap Malondialdehid Tikus Putih (Rattus Novergicus) Yang Diinduksi Karbon Tetraklorida

Suryanita Suryanita, Hasma Hasma

Abstract

Free radicals are any molecule or atom that is unstable because it has one or more unpaired electrons. Free radicals that are formed in the body will produce new free radicals through a chain reaction which eventually increases in number and attacks the body's cells so that tissue damage will occur, therefore antioxidants are needed from outside to neutralize free radicals, the salute is grapefruit peel (Citrus maxima Merr.) This study aims to determine the antioxidant activity of the ethanol extract syrup of grapefruit peel against levels of Malondialdehyde (MDA) blood plasma of male white rats induced by carbon tetrachloride (CCl4). The stages of this study were the first to make a skin extract syrup. grapefruit peel containing 1% extract (F1), 2% extract (F2), 3% extract (F3), and without grapefruit peel extract (F4) as negative control; Furthermore, testing the stability of the syrup preparations before and after storage was accelerated for 12 cycles, and finally the syrup with the best stability was tested for its lipid peroxidation inhibition activity in vivo with blood plasma MDA levels in rats induced by Carbon Tetrachloride. The results showed the results of the syrup stability test. indicates that F3 has the best stability. As for the in vivo test results, F3 showed a decrease in the MDA level of blood plasma in rats which was significantly different from the negative control. The grapefruit peel significantly decreased the MDA level of the rat blood plasma with a value p<0.05.

Keywords : Free radicals, Grapefruit Peel, Antioxidant, Syrup Preparation, Malondialdehyde (MDA)

Full Text:

PDF (Full Text)

References

Parwata, A.O.I. 2016. Kimia Terapan. Universitas Udayana. Denpasar

Tuminah, S. 2010. Pencegahan kanker dengan antioksidan. Cermin Dunia Kedokteran 122:21-23.

Dean, R.T., Fu, S., Stocker, R., and Davies, M.J.2009. Biochemistry andPathology of Radical-Mediated Protein Oxidation. Biochem. J. 324 :1-18

Choi, S.Y., Ko, H.C., Ko, S.Y., Hwang, J.H., Park,J.G., Kang, S.H., Han,S.H., Yun,S.H., andKim, S.J. 2007. Correlation between Flavonoid Content and the NO Production Inhibitory Activity of Peel Extracts from Various Citrus Fruits.Biol. Pharm. Bul, 30:772-778.

Chowdhury , M. R., Hasan, M. A. T., Sagor, N., Tabassum, M. A., Potol, H., Hossain, A., and Alam. 2015. Supplementation of Citrus maxima Peel Powder Prevented Oxidative Stress, Fibrosis, and Hepatic Damage in Carbon Tetrachloride (ccl4) Treated Rats. Hindawi Publishing Corporation, 2015 (10)

Rafsanjani, Kabul, M., dan Putri, W.D.R. 2015.Characteristic of Pamello Fruit Peel Extract Using Ultrasonic Bath (Study Of Solvent and Extraction Time).Malang : Jurusan Teknologi Hasil Pertanian, FTP Universitas Brawijaya Malang

Marks, D.B., Marks, A.D., and Smith, C.M. 2009. Biokimia Kedokteran Dasar. (Brahm U.Pendit, Pentj). EGC : Jakarta

Hamid, A.A.,Aiyelaagbe, O.O., Usman, L.A., Ameen, O.M., and Lawal,A. 2010. Antioxidant : its Medidal and Pharmacological Applications.African Journal of pure and applied chemistry, 4(8) : 142-151

Zuhra, C. F., Juliati B.R., Tarigan, and Herlince S. 2008. Aktivitas Antioksidan Senyawa Flavonoid Dari Daun Katuk (Sauropus androgunus (L) Merr.). Jurnal Biologi Sumatera : 7-10.

Ditjen POM. 1995. Farmakope Indonesia Edisi IV. Jakarta : Departemen Kesehatan Republik Indonesia

Murray,R.K., Granner, D.K, Mayes, P.A., adn Rodwell, V.W. 2003. Harper’s Illustrated Biochemistry Twenty-Sixth Edition. New York: McGraw-Hill

Rowe, R.C., Sheskey, P.J., Quinn, M. E. 2009. Handbook of Pharmaceutical Excipients, 6th Eddition. ThePharmaceutical Press: London

Sayuti, K., and Yenrina, R. 2015. Antioksidan Alami dan Sintetik. Andalas University 1Press: Padang

Jeon, T.I., Hwang, S.G., Park, N.G., Shin, S.I., Choi, S.D., and Park, D.K. 2003. Antioxidative effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats.Toxicology 187:67-73.

Shanmugasundaram, P., and Venkataraman, S.J. 2006. Hepatoprotective and antioxidant effects of Hygrophila auriculata (K. Schum) Heine Acanthaceae root extract . Ethnopharmacol, 104: 124-128.

DOI: https://doi.org/10.32382/medkes.v16i1.1836