ANALYSIS OF DIFFERENCES IN ERYTHROCYTE MORPHOLOGY IN K3EDTA AND Na2EDTA BLOOD CLOTS BASED ON TIME SAMPLE STORAGE

Abstract

Blood is a liquid system that combines blood plasma and blood cells. Blood cells consist of three types, namely erythrocytes, leukocytes and platelets. For analitical check up, anticoagulant is needed to prevent blood clotting by binding (chelation) or precipitating (precipitation) calcium, or by inhibiting the formation of thrombin needed to convert fibrinogen to fibrin in the freezing process. Check up using EDTA blood tests must be done immediately, if forced to postpone it should be noted the storage time limit for each check up. To make peripheral blood smear preparations can be used EDTA blood stored at least 2 hours at room temperature. There are two types of EDTA salts, namely sodium and potassium. The purpose of this research was to find out the comparison of the erythrocyte morphology in blood smear K3EDTA and Na2EDTA based on storage time variations. The type of research conducted is laboratory experiments. Data analysis used t test for erythrocyte form and description for erythrocyte size and color. The results of the t test between the forms of blood cationation Na2EDTA and K3EDTA chronology obtained significance values (2 tailed) 0.613> 0.05 (p> α) so that it can be concluded that H0 is accepted and Ha is rejected which means there are differences but not meaningful, but can be seen from the mean Na2EDTA (20,1667)> K3EDTA (17,4167) which means the average Na2EDTA crenation is higher than the K3EDTA blood sample. There is no significant to morphological changes between the blood sample of Na2EDTA and K3EDTA. There is storage time effects on the erythrocyte shape but there is no effect on the size and color of erythrocyte.
Keywords: Edge Blood Smear, Na2EDTA and K3EDTA, erythrocyte morphology