The Comparison Results of Examination of Platelet Counts using EDTA Blood and Sodium Citrate Using Hematology Analyzer

Artati Artati

Abstract


The Platelet count is one of the most important examinations for various cases both involving hemostasis and other cases, including establishing the diagnosis, evaluating the outcome of therapy or the course of a disease, determining the prognosis, and assessing the severity of the disease. So in doing so, we must pay attention to the pre-analytic, analytic, and post-analytic stages. One of the factors that influence platelet count is the use of anticoagulants, which sometimes the use of EDTA anticoagulants can cause cases of pseudo thrombocytopenia, so it is necessary to re-draw blood using Sodium Citrate anticoagulant. This study aimed to determine the Comparison of Platelet Count Examination Results using EDTA blood and Sodium Citrate is a Hematology Analyzer. This study applied a descriptive with a sample of 7 patients with a purposive sampling technique. This research was carried out at the Prodia Manado laboratory from April to May 2020. Data were obtained and analyzed using paired sample t-test. The research findings showed an average number of platelets using EDTA blood of 92.71 (* 10³ / µL) and Sodium Citrate 197.71 (* 10³ / µL) with a (p-value) sig (2-tailed) of 0.001 < alpha value (0.05), thus it can be concluded that there is a significant difference between the number of platelets using EDTA blood and Sodium Citrate in the Hematology Analyzer.

Keywords: Thrombocyte, EDTA, Na Citrate, Hematology Analyzer



Full Text:

PDF

References


A’Malina LO, 2018. Perbedaan Penggunaan Antikoagulan EDTA Konvensional dan EDTA Vacumtube pada Jumlah Trombosit Metode Hematology Analyzer. KTI. Universitas Setia Budi

Brain B.J, 2015. Hematologi Kurikulum Inti. Jakarta : EGC

Brunner & Suddarth. 2013. Buku Ajar Keperawatan Medikal Bedah Edisi 8 volume 2. Jakarta : EGC

Corwin Elizabeth J, 2009. Buku Saku Patofisiologi. Edisi ke tiga. Jakarta : EGC

DEPKES, 2008, Pedoman Praktik Laboratorium Yang Benar (Good Laboratory Practice)

Gandasoebrata, R. 2013. Penuntun Laboratorium Klinik, Cetakan ke-15. Jakarta : Dian Rakyat

Handayani W, Haribowo, Andi Sulistyo, 2008. Asuhan Keperawatan pada Klien dengan Gangguan Sistem Hematologi. Jakarta : Salemba Medika

Hoffbrand AV ; Pettit ; PAH Moss, 2013. Kapita Selekta Hematologi (Essential Heamatology), Jakarta : Penerbit Buku Kedokteran EGC

http://www.atlm.web.id/2016/12/jenis-jenis-warna-tabung-vacutainer-dan.html Diakses pada 25 Januari 2020

http://www.biomagz.com/2015/08/sel-darah-merah-putih-dan-keping-darah.html. Diakses pada 15 Januari 2020

https://www.google.com/search?q=tabung+vacutainer+heparin. Diakses pada 25 Januari 2020

https://www.google.com/tabung+antikoagulan=firefox-mg). Diakses pada 25 Januari 2020

Imandalis, 2012. Kelebihan, (hptt;//imandalis. blogspot.co.id/ 2012/04/ hematologi –autoanalyzer –kelebihan –dan.html) diaskes pada tanggal 23 januari 2020

Jumailia R, 2017. Perbandingan Jumlah Hitung Trombosit pada Apusan Darah yang Menggunakan Darah EDTA dan Darah Natrium Sitrat. Skripsi. Surakarta : Fakultas Ilmu Kesehatan. Universitas Setia Budi

Kalma, Bakhri S, Naim N, Umar B, Hurustiaty, 2015. Flebotomi Terapan. Makassar

Kiswari R, 2014. Hematologi dan Transfusi. Jakarta : Erlangga Famedia.

Kosasi EN & Kosasi AS. 2008. Tafsiran hasil pemeriksaan Laboratorium klinik. Karisma Publishing group. Tangerang

Kurniawan LB, 2014. Konfirmasi Apusan Darah Tepi untuk Pseudotrombositopenia CDK-217/Vol 41 no 6

Marpiah S, Santosa B, Ariyadi T, 2017. Pengaruh Darah k3EDTA Terhadap jumlah Trombosit menggunakan Alat Autometik Hematology Analyzer.

Nugraha Gilang, 2017. Panduan Laboratorium Hematologi Dasar. Jakarta Timur : CV Trans Info Media

Pramytasari EDP, Krihariyani D, Eahyuni S. 2016. Sitrat Estimasi Perhitungan Jumlah Trombosit Sampel Darah Terhadap Ethylene Diamine Tetraacetic Acid (EDTA). ISSN : 2302-3635. Vol 5 no 2

Rahma L dr. 2017 http:// drlestarirahmah. blogspot.com/2017/07 /trombositosis. html. Diakses 17 Februari 2020

Sacher RA dan Mcpherson RA, 2012. Tinjauan Klinis Hasil Pemeriksaan Laboratorium. Jilid Kesebelas. Jakarta : Buku Kedokteran EGC

Sianipar NB, 2014. Trombositopenia dan Berbagai Penyebabny. CDK-217/ vol 41 no 6

Sorfo A.S.M, 2012. Darah. Yogyakarta : Pustaka Pelajar

Sysmex, 2017. XN-Series Training Class Prodia

Trisakti, Dinda, 2015. Hematologi Analizer. (https://www.scribd.com/doc/29249497/hematologi-analizer-docx). Diakses 20 Januari 2020.




DOI: https://doi.org/10.32382/uh.v3i1.2734

Refbacks

  • There are currently no refbacks.